Birmpa A, Kouroupis G, Kalogeropoulos K, Kokkinos P, Kritsonis P and Vantarakis A
Salmonella spp., Listeria monocytogenes and viruses constitute major concern for public health. It is known that RTE foods are consumed raw, thus they are often associated with outbreaks of food poisoning. In the present study, the effectiveness of a LAMP fully automated platform able to detect foodborne pathogens in less than an hour, was evaluated. Food samples were inoculated with known pathogens, such as S. enteritidis, L. monocytogenes and hAdV 40/41. With LAMP assays pathogens can be detected shortly and without the need of sophisticated equipment. The results of the present study were then compared to those of the LAMP assays detected by a Light Cycler Roche platform which is a real-time PCR instrument and were in concordance. The developed LAMP platform that is presented in this study could become a valuable, robust, innovative, powerful, cheap and fast monitoring tool which can be extensively used for routine analysis and screening of contaminated foods by the food industry and the Public Health Authorities
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