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Effect of Semen Diluents, Dilution Rates, and Storage Periods on Spermatozoa Motility of Different Varieties of Guinea Fowl

Abstract

Keerthy AJ, Omprakash AV, Churchil RR and Hudson GH*

Twelve number of healthy male guinea fowls from each variety of pearl, white, white breasted and lavender were selected based on the phenotypic characters and trained for semen collection by abdominal massage technique. Pooled semen from each variety, were diluted with the lakes semen extender, Modified Beltsville poultry semen extender, and Beltsville poultry semen extender each in the ratio of 1:3, 1:4 and 1:5. The spermatozoa motility was assessed at 0,1,2,4 and 6 hours of storage. Maximum percent motility of 83.33, 86.67 and 79.19 were observed in the semen of pearl, white breasted and lavender varieties of guinea fowl diluted Beltsville poultry semen extender in the ration 1:4 at maximum storage period. Maximum spermatozoa motility in white guinea fowl variety was observed with the semen diluted with BPSE at the ratio of 1:3 (81.67). The current study will be of immense useful for the selection of semen diluents for better fertility through artificial insemination in guinea fowls.

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