生物医学与药物科学杂志

Evaluation of Antioxidant and Anti-Inflammatory Activity of Methanolic and Aqueous Extract of Arisaema propinquum Schott Rhizomes

Abstract

Prince Ahad Mir, Mohd Akbar Dar, Roohi Mohi-ud-din and Ghulam Nabi Bader

Objective: The present study was carried out to evaluate the antioxidant and in-vitro anti-inflammatory activity of Arisaema propinquum Schott rhizomes.

Methods: The alcoholic and aqueous extracts were first tested for phytochemical screening. In-vitro antioxidant activity of extracts was evaluated by DPPH free radical (1, 1-diphenyl-2-picryl-hydrazyl) scavenging assay, reducing power method and hydrogen peroxide (H₂O₂) scavenging assays. To investigate the in-vitro anti-inflammatory activity of the extracts HRBC (Human Red Blood Cell) membrane stabilization method and percentage protein denaturation inhibition method was used.

Results: Phytochemical screening of the extracts reveals the presence of alkaloids, carbohydrates, cardiac glycosides, coumarins, proteins, amino acids, phenols, tannins, flavonoids, saponins, steroids and terpenoids. The total phenolic and flavonoid content in methanolic and aqueous extract was found to be 554.06 ± 131.60 and 425.21 ± 101 mg/g Gallic Acid Equivalents (GAE) and 324.71 ± 99.84 and 225.56 ± 42 mg/g Rutin Equivalents (RE) respectively. The methanolic extract showed potent percentage inhibition of DPPH and H₂O₂ scavenging activity as compared to standards rutin and ascorbic acid. Both the extracts showed good membrane stabilizing as well as protein denaturation activity when compared with the standard Indomethacin.

Conclusion: The results of the present study demonstrate the antioxidant and anti-inflammatory potential of the methanolic and aqueous extracts of Arisaema propinquum Schott rhizomes. However, it is necessary to isolate and purify the active principles responsible for pharmacological activity to determine its mechanism of action.The results of the present study demonstrate the antioxidant and anti-inflammatory potential of the methanolic and aqueous extracts of Arisaema propinquum Schott rhizomes. However, it is necessary to isolate and purify the active principles responsible for pharmacological activity to determine its mechanism of action.