Falmata Kyari, Albert Wulari Mbaya, Abdullahi Abubakar Biu, Lawan Adamu and Ali Abbagana Benisheik
Trypanosoma evansi is a parasitic protozoan that allows surra disease to spread across subtropical and tropical regions of the universe. The objective of the present study was to evaluate the diagnostic techniques using experimentally infected (dromedary) Camels. Thirty-five apparently healthy adult camels of both sexes were used in this experiment. The camels were administered intravenously with 0.5 ml of blood infected with T. evansi via the lateral abdominal vein. Blood samples were obtained from the camels on days 0, 4, 8, 12, 16, 20, 24, and 28 after infection to evaluate the detection rate of the various diagnostic tests. The receiver operating characteristic curve (ROC-Curve) was used for the evaluation of the sensitivity of the diagnostic techniques; BCT=50.0%, CATT/T. evansi=71.84%, MI=61.63%, PCR=81.43%, TBS=68.37% and WBF=68.37% respectively. On day 4, no T. evansi were detected for the following techniques; WBF, BCT, TBS, and MI. While the detection rate of the CATT/T. evansi and PCR techniques were 70% and 100% respectively. The detection rate of WBF, TBS, BCT, MI, CATT/T. evansi and PCR on day 24 of the experimental analysis were not statistically different. The prevalence rate of WBF was significantly lower (p>0.01) compared with PCR (WBF=60%, CI=44%, 74%; PCR=91%, CI=78%, 97%). The association between the different diagnostic techniques and detection of T. evansi infection in experimentally infected Camels was strong and significant (Chi-squared=13.720, degree of freedom (df)=5, p=0.0175). In conclusion, PCR has the highest detection rate and is the most sensitive technique. The association between the different diagnostic techniques and detection of T. evansi infection in experimentally infected Camels was strong and significant.
分享此文章
English 
Spanish 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi 