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Multiplexed Quantification of Metabolites with MISSILE

Abstract

Jones DR, Wang X, Shaw T, Cho JH and Peng J

We tested a strategy for multiplexed (4-plex) quantification of metabolites using the MISSILE identification method with liquid chromatography coupled to tandem mass spectrometry. We applied this methodology to study the metabolic effect of the proteasome inhibitor and chemotherapeutic drug Bortezomib in yeast cells. Using JUMPm software version 1.1 we simultaneously identified and quantified 95 metabolites across four experimental conditions and found that Bortezomib increased the accumulation of dipeptides but decreased the levels of specific lipid molecules (e.g. phosphtidylethanolamines) in a dose-dependent manner. This method combines metabolite identification and quantification, making untargeted metabolomics experiments more informative.