组织科学与工程学报

Spontaneous Chondrocyte Maturation on 3D-Chitosan Scaffolds

Abstract

Serafim M Oliveira, Gloria Turner, Simone P Rodrigues, Mário A Barbosa, Mani Alikhani and Cristina C Teixeira

The endochondral ossification mechanism provides promising new insight into potential techniques for repairing bone defects. The methods for replicating endochondral ossification in culture, although diverse, have yet to identify a procedure that reflects natural conditions, while satisfying the practical, experimental needs for efficiency and maneuverability. In this study, we present a novel approach that addresses all of these concerns. Coordinated proliferation and differentiation of chondrocytes in a transient cartilage is required for endochondral bone growth, but the mechanisms and pathways that control these processes are not completely understood. For lack of alternative methods, chondrocyte cultures are commonly pushed to differentiation with growth factors and supplements, like retinoic acid. We have developed a tissue culture model, using a 3D-chitosan sponge, which supports the proliferation and differentiation of chondrocytes, without exposure to supplements, and which matures into a near replica of the growth plate.