Ana Paula Franco Lambert, Dinara Moura, Aline Fraga Zandonai, Mariana Assis Lemos, Jeremiah Lubianca, Christian Viezzer, Joao Alvaro Souza da Silva, Diego Bonatto, Denise Cantarelli Machado and Joao Antonio Pegas Henriques
The development of a clinically translatable method of engineering with adipose-derived adult stem (ADAS) for reconstruction requires investigation of several components. The differentiation of ADAS cells into neuronal cells has been reported by several groups. The stringent maintenance of genomic stability in adult stem cells via anti-stress defenses and DNA repair mechanisms is particularly important because any genetic alteration can compromise the genomic stability and functionality of the cell. The main objective of this data was to examine some parameters related to DNA damage in cells submitted to the neural differentiation protocol and to understand if DNA damage can be associated to cell differentiation. The comet assay, micronucleus tests, and the cell viability assay were utilized to observe ADAS cells treated with neural induction medium. The results of our genotoxicity assays suggest that increased DNA damage observable by the comet assay was induced by neural differentiation. Emerging findings suggest that DNA damage; telomerase and DNA repair proteins play important roles in neurogenesis developing. Surprisingly we obtain evidence for an association between DNA damage and neuronal-like differentiation and hypothesize that during neural differentiation DNA damage will recruit telomerase TIP60 and MCM3, where they may function in DNA repair, chromatin remodeling and limiting DNA replication.
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