生物工程与生物医学杂志

Study objective: The aim of our study was to compare the effectiveness of EDTA cleaning gel to 17% when combined with sodium hypochlorite (NaClO) to 2.5% during canal preparation, with respect to the use of NaClO alone. By using two preparation techniques, the manual technique using manual files steel and continuous rotation with the Hero-shapers system.
Materials and methods: The study was conducted in vitro on infected teeth, freshly extracted single rooted. All teeth were carefully cleaned and sectioned at the JCD.
On each tooth, a bacteriological sample was taken before and after treatment canal with sterile paper points and analysed according to microbiological standards.
The study was conducted on a total of 84 teeth randomly divided into 4 groups of 21 teeth each as follows:
The first group was prepared manually with steel instruments and received sodium hypochlorite at 2.5% as irrigating; the 2nd group was manually prepared with the instruments steel and received sodium hypochlorite at 2.5% associated with EDTA gel at 17% as irrigating; the 3rd group was prepared with rotary instruments Hero Shaper and received sodium hypochlorite at 2.5% as irrigating; the 4th group was prepared with rotary instruments Hero Shaper and received sodium hypochlorite at 2.5% associated with EDTA at 17% as irrigating. The results of bacteriological analysis were processed using Epi Info 3.3.2 software, retained significance index p <5%.
Results: 23% of germ eradication for the teeth of the first group, 56% for the 2nd group, 47% for the 3rd group and 68% for the 4th group.
Conclusion: The use of a chelating agent undeniably improves the quality of cleaning of the root canals, compared with the use of only sodium hypochlorite.

A tumour invasion model has been developed to examine the influence of diffusible matrix metalloproteinases (MMPs) and the mediated proteolysis of non-diffusible, membrane type MMPs (MT-MMPs) on tumour growth and morphology via haptotaxis. Our results are the first to explore the influence of localized degradation of extracellular matrix (ECM) by MT-MMPs on the morphology of tumours growing in nutrient-rich and nutrient-poor microenvironments. Two-dimensional numerical simulation, using a level-set-based tumour host interface-capturing method, reveals that haptotaxis due to ECM degradation by MMP causes greater instability than with ECM degradation by MT-MMP in low-nutrient environments, even at low proliferation rates; whereas the resulting morphologies are similar for high apoptosis rates. Our simulation results show that while haptotaxis leads to completely different tumour growth rates and morphologies depending on proliferation and apoptosis rates in low-nutrient environment, there are no significant variations when we compare the haptotaxis due to ECM degradation by MMP and MT-MMP, except for low proliferation rates. Focusing on the differences between MMP and MT-MMP mediated effects; our study has important implications in MMP-target validation and MMP-inhibitor-drug development for anti-cancer clinical trials.

Thesis: An attempt to analyse the mechanism of cardiovascular disease (CVD). It is now believed that most heart disease of unknown etiologic and mechanism, with the CVD mortality is very high. Why theoretical medicine for over 100 years has such limited success? What is the true reason, for example, venous plethora?

Methods: Finding information in the literature, participation in medical conferences in Russia (2012-2016), as well as in Australia, Brisbane "Arrhythmia-2016", correspondence with scientists, discussions with leading Russian cardiologists, publication of my original articles.

Results: I was able to show that in modern theoretical cardiology there are significant errors. Our group with an instrument "Cardiocode" were able to confirm the presence of large arteriovenous anastomoses (AVA) in otherwise healthy people. The sudden opening of the lumen AVA causes a sharp drop in blood pressure.

When closing AVA, usually a few seconds, the blood pressure increases again. Such manipulations AVA, apparently provided by nature to protect the blood vessels from the peak pressure values. It turns out that the longterm "wrong" operation AVA, especially when an unhealthy lifestyle usually leads to two major problems.

First problem: The periodic disturbances of heart rhythm, sometimes with fatal outcome, due to excitation of the heart by means of a pulse of mechanical waves running through the contour of the vessels with the return of their heart. The second problem is: violation of "metabolism" of the prolonged increase in venous pressure as a systemic and local. This paper attempts to study the causes of venous plethora and diseases of the pelvic organs in humans.

Conclusions: I believe that, taking into account the vast experience of the modern official medicine, taking into account the large number of clinical observations, and using in the near future, the theory proposed by me might be able to make a breakthrough in the treatment of cardiovascular disease, including in the treatment of venous plethora.

In the light of all the medical research that is being pursued in our world today to find the answers to the ills, diseases, health problems, and accidents to which humankind is prone; it seems strange that very little time and funding is at hand to research how falls can be prevented. It is time we begin to find some answers. By working with a person’s gait and discovering what happens to the roll, pitch, and yaw as a person walks we can begin to develop skills to use in rehabilitation so that an individual can walk more safely; skills that can correct the roll, pitch and yaw when a deviation is found that causes difficulty when walking. What we see here in this paper is only the beginning.

The first aim is to establish a standardized method for stability testing. A second aim is to determine a method of analysis of the recorded data.

A series of tests were conducted for the benefit of residents of a retirement community. The long term goal is to aid in fall prevention.

An inertial sensor was used to obtain real time data during the tests, and these data were recorded and saved for later evaluation.

In the present study, sunlight irradiation induced strategy for the rapid green synthesis of silver nanoparticles (AgNPs), is reported for the first time using glycolipid bio-surfactant. On exposing a mixture of silver nitrate solution and glycolipid bio-surfactant in the ratio 3:1 to sunlight, stable silver nanoparticles were obtained within few minutes. The glycolipid bio-surfactant acts as both reducing and stabilizing agents in the synthesis of nanoparticles. The nanoparticles synthesized were characterized using UV–Vis spectrophotometer, scanning electron microscopy and FTIR. Synthesized nanoparticles were in the range of 70-90 nm with spherical shape. The absorption spectroscopy indicates the band gap of silver nanoparticles was 3.4 eV. Further, the antibacterial activity assay of the synthesized nanoparticle exhibits as potential antibacterial agents. Hence, the present study demonstrates that biologically synthesized nanoparticle could be employed for developing antibacterial drug.