组织科学与工程学报

Complex multi-lamellar biocomposite structure of Intervertebral Disc (IVD) imparts flexibility between adjacent vertebras, as well as allows transmission of loads from one vertebra to the next along the spine. The disc has a 15- 25 concentric layered laminate structure; each layer is reinforced by collagen fibers which are aligned at approximately 30 degree angle in successive layers with respect to the transverse plane of the disc. This fibrous organization is critical to the proper biomechanical functioning of the disc, such as to convert compressive force to lateral force, to withstand extrinsic tensile stresses (circumferential, longitudinal and torsion). As a result spine becomes flexible to bend and twist. With the regular aging the disc gets dried up lost its flexibility and biomechanical elasticity. That’s why we need tissue engineering of that degenerated tissue to make a proper ailment of that body part by the help of some textile fibers like silk- hydrogel, CMC, PVA- collagen, PGA – chitosan composites. The synthetic polymer has shown great promise for easiness of production, variability in properties and biodegradability and biocompatibity and non-immunogenic response inside the human spinal body for the novel cause of removal and restoration of degenerated human intervertebral disc.

Background: Various surgical methods have been designed to avoid the necessity of using periosteum, i.e.
an operative weak point of autologous chondrocyte implantation (ACI) which is a representative method for treating
articular cartilage injury. This study was performed in the attempt to develop a simple, injectable type of ACI to in
order facilitate the surgical process.
Methods: Seven of 10 dogs were used for the injectable type ACI using fibrin, while the remaining three dogs
were used for implantation according to the previous surgical method using periosteum. The left knee of each dog
was incised in order to expose the medial femoral condyle, and a circular defect was then made to 5mm in diameter
on the articular cartilage of the exposed medial femoral condyle so that chondrocyte implantation using periosteum
and the injectable chondrocyte implantation using fibrin glue could both be performed. At week 12 following surgery,
the cartilage was observed and compared histologically with normal articular cartilage.
Results: The surface of the cartilage newly generated at week 12 was very smooth and even, and it was also
seen that the entire area was completely regenerated. Through the histological evaluation, IHC test, and electron
microscope pictures, it was verified that collagen type II was normally expressed and that the ultrastructure of the
regenerated tissue showed the normal cartilage properties.
Conclusion: Gel-type ACI using fibrinfor articular cartilage defects of the knee, appears to be an effective
method for the regeneration and growth of cartilage and also has many potential surgical advantages.

Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (aPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.
Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental groups received PL/PG on the surgery site. tPA, PAI-1, IL-6 and active TGFb1 were analyzed at given time points postoperatively in peritoneal lavage. Adhesions were evaluated after seven days. Significant differences were considered to be p<0.05.
Results: At a few individual time points small differences were seen between the groups (control and experiment)
comparing levels of tPA, PAI-1, IL-6 and active TGFb1. When comparing levels of substances from all time points no statistical differences were seen between the groups as a total. Adhesions were significantly decreased on day 7, p=0.002.
Conclusion: Despite significant reduction in adhesions PL/PG administered intraperitoneally as an anti-adhesion agent locally on surgically traumatized area does not seem to affect the normal process of peritoneal healing.

Myelodysplastic syndromes (MDS) are hematological diseases with a wide clinical spectrum of manifestations. We present a 66 year old patient who was referred to our department with an acute submandibular growth that was first noticed 2 weeks ago. It hasn’t responded to standard treatment. Multiple studies were performed, and they revealed in filtrate of mielodisplastic cells, refractory anemia with excess blasts in transformation (RAEB-T) subtype according to the FAB classification. Combined chemotherapy couldn’t stop the disease’s progression and the patient died from a pulmonary infection 3 months after the onset of symptoms. Leukemic infiltration of the submandibular gland in mielodisplastic syndrome hasn’t been previously described in literature, so this seems to be the first reported case of extra medullary hematopoiesis resulting in an acute submandibular tumor in a patient with REAB-T.

Biobanking, the archiving of biological specimens from donors for use in research, has become an important resource for healthcare research, medical care, and drug discovery. The kinds of questions being addressed in contemporary medical research depend upon the availability of biological tissue samples along with associated clinical data including medical, genealogical, and lifestyle information.