组织科学与工程学报

Impaired elastic matrix remodeling occurs in reproductive tissues, after vaginal delivery. This has been linked to development of pelvic organ prolapse (POP), for which there currently is no pharmacologic therapy. Hyaluronan oligomers and transforming growth factor beta 1 (termed elastogenic factors, EFs), have been shown to significantly enhance tropoelastin synthesis, elastic fiber assembly, and crosslinking by adult vascular smooth muscle cells(SMCs). The goal of this study was to ascertain, if these factors similarly improve the quantity and quality of elastic matrix deposition by vaginal SMCs (VSMCs) isolated from lysyl oxidase, like–1 knock out (LOXL1 KO) mouse model of POP. Cells isolated from whole vagina of a LOXL1 KO mouse (multiparous, stage 3 prolapse) were cultured and identified as SMCs, by their expression of various SMC markers. Passage 2 vaginal SMCs (VSMCs; 3×104/10 cm2) were cultured for 21 days, with EFs. Cell layers and spent medium aliquots were assessed for elastin content and quality. EF-treated VSMCs proliferated at a similar rate to untreated controls, but synthesized more total elastin, primarily in the form of soluble matrix elastin. Elastin mRNA was also increased compared to controls. The elastic matrix was significantly denser in EF-treated cultures, which was composed of more mature, non-interrupted elastic fibers that were absent in controls. The results are promising towards development of a therapy, to enhance regenerative elastic matrix repair in post–partum female pelvic floor tissues.

In this study we investigate for the first time the biomedical potential of using a membrane made from anodic aluminium oxide (AAO) for culturing the Madin-Darby Canine Kidney (MDCK) epithelial cell line. Nano-porous aluminium oxide membranes exhibit interesting properties such as high porosity, which allows the exchange of molecules and nutrients across the membrane and can be made with highly specific pore sizes that can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The cellular response and interactions of the MDCK cell line with the synthesised nano-porous AAO membrane, a commercially available membrane and a glass control were assessed by investigating cell adhesion, morphology and proliferation.