生物加工与生物技术杂志

Characterizations of Bacterial Cellulose Producing Strain Gluconoacetobacter hansenii CGMCC3917

Abstract

Dehui Lin, Nan Wang, Zhixi Li*, Yan Li

Gluconoacetobacter hansenii CGMCC3917 (M₄₃₈), isolated from inoculums of strain J₂ treated by high hydrostatic pressure, has strong ability of producing cellulose as more than three times as that of its initial strain J₂. In this paper, in order to further study the effects of high hydrostatic pressure treatment on characterizations of strain J₂ on the basis of previous study, properties of these two strains were examined and compared. The results indicated that the mutant strain M₄₃₈ and its initial strain J₂ had different Phenotypic Characterizations in liquid seed medium. The fermentation parameters showed that cell growth rate of strain M₄₃₈ was relatively higher than that of strain J₂, namely, residual sugar, residual nitrogen and acidity of strain M₄₃₈ were less than that of strain J₂. Furthermore, water holding capacity (WHC) and water release rate (WRR) of bacterial cellulose (BC) membranes produced by M₄₃₈ were both better than those of BC membranes produced by J₂. However, SEM imagines suggested that there was no evidence difference in microstructure of BC membranes. Additionally, FT-IR also showed no difference between BC membranes produced by strain M₄₃₈ and its initial strain J₂.