Natalia Perez
Isolation and purification techniques are fundamental in natural products chemistry, as they enable the extraction of bioactive compounds from complex biological matrices. This paper provides a comprehensive overview of contemporary methods employed in the isolation and purification of natural products. It discusses traditional techniques such as solvent extraction, liquid-liquid partitioning, and chromatographic methods including column chromatography, high-performance liquid chromatography (HPLC), and thin-layer chromatography (TLC). Additionally, it explores advanced methodologies such as Supercritical Fluid Extraction (SFE), molecularly imprinted polymers (MIPs), and preparative chromatography. The advantages, limitations, and applications of each technique are analyzed to offer insights into their role in the development of pharmaceuticals, nutraceuticals, and other valuable products. The review underscores the importance of combining multiple techniques to achieve high purity and yield, and it highlights recent innovations and future directions in the field.
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