生物传感器与生物电子学

L-Ascorbic Acid Biosensor Based on Immobilized Enzyme on ZnO Nanorods

Abstract

Z. H. Ibupoto, Syed M. Usman Ali, K. Khun and M. Willander

In this present research, we have grown the well aligned, perpendicular to the substrate and highly dense ZnO nanorods on the gold coated substrate by using the hydrothermal growth method. The ZnO nanorods were functionalized by immobilizing the ascorbate oxidase enzyme with cross linking molecule 3-glycidoxypropy1trimethoxysilane (GPTS).The electrochemical measurements were carried out through using potentiometric technique, we observed that the output response exhibited by the biosensor to the wide linear dynamic range concentration of L-ascorbic acid from 1×10 -6 to 5×10 -2 M with good sensitivity of 32 mV/decade. Moreover the biosensor showed excellent fast response time less than 10 seconds, better selectivity, repeatability, reproducibility and no significant interference to the common interfering ions such as potassium K +1 , sodium ions Na +1 , calcium ions Ca +2 , glucose, fructose, and zinc ions Zn +2 etc. except slight interference with copper Cu +2 ions.