生物分析与生物医学杂志

Pharmacokinetics of Casiopeína IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity

Abstract

Cañas-Alonso RC, Fuentes-Noriega I and Ruiz-Azuara L

Casiopeína IIgly is a mixed chelate coordination complex with copper core that have demonstrated high antineoplas- tic activity in vitro and in vivo . In the present work, a de- veloped a nd va lida ted method for mea surement of Casiopeína IIgly in beagle dog and its application in a phar- macokinetic study is reported. The analyte was isolated from blood by solid-phase extraction using Strata X cartridges. The analysis was carried out on a Synergy Polar-RP col- umn (30 X 2.0 mm) using an isocratic elution and MeOH/ HFBA 0.1% (4:6) as the mobile phase. An Agilent LC/ MSD Trap VL equipped with an ionization electrospray source, was operated in selective ion storage (SIS) using stable ion [Cu (II) (F 7 C 3 COO) 4,7-dimethyl phen ] + with 484 m/z for quantification result of ESI reaction between Casiopeína IIgly and HFBA. The method demonstrated to be linear (r = 0.9992) in the range from the 0.1 to 15 μ g/ml with a limit of detection (LOD) of 50 ng/ml. All the param- eters of validation such as selectivity, accuracy, precision and recovery were within the required limits. Pharmacoki- netics assay was carried out at 2 doses, indicated a high elimination rate.