Ali Tafaghod
The study of single-cell heterogeneity is a fundamental aspect of understanding complex biological systems. This article explores the transformative capabilities of quantitative Imaging Flow Cytometry (qIFC) in unraveling the intricacies of single-cell analysis. We discuss the principles, instrumentation, and data analysis techniques associated with qIFC, emphasizing its capacity to provide quantitative and spatial information at the single-cell level. This technology enables comprehensive investigations into cell populations, revealing hidden phenotypic diversity, dynamic responses, and subcellular features. The integration of qIFC is poised to advance our knowledge of cellular biology, immunology, and disease mechanisms.
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