计算机科学与系统生物学杂志

Homology Modelling of Hypoxanthin–Guanine Phosphoribosyltransferase, Enzyme Involved in Salvage Pathway of Purine Metabolism

Abstract

Jyotsna Choubey, Ashish Patel, Sheetal Khatri, Ruby Dewangan, Shailendra Kumar Gupta and M.K. Verma

Hypoxanthin-guanine phosphoribosyltransferase (HPRT) is responsible for catalyzing a reaction which breaks down uric acid in the body. The lack of this enzyme creates a build-up of uric-acid, leading to a neurological problem, joint pain and kidney problem. The condition is called Lesch-Nyhan Syndrome. A comparative modelling of the protein Hypoxanthin-guanine phosphoribosyltransferase is performed using the modeling program modeller9v3.The following structure validation programs PROCHECK, PROSA, VERIFY3D and WHATIF are used. Descret Optimized Protein Energy (DOPE), is a statistical potential used to asses homology model in protein structure prediction. Predicted model can be useful to develop new inhibitor against Lesch-Nyhan Syndrome. CASTp can be used to study surface features and functional regions of proteins.