Zhenhua Gong, Kang Zhang, Lei Li, Hongwei Wang, Yuan Qiu, Inping Li, Guangyu Hou, Jianmin Yu, Jianlin Wang and Hu Shan
In this study, Marek’s disease(MD) vaccines including herpes virus of turkeys (HVT) vaccine, attenuated serotype 1 Marek’s disease virus (MDV) CVI988/Rispens vaccine and nonpathogenic serotype 2 MDV (SB1 strain) culture were used individually or jointly to immunize 1-day-old specific pathogen free (SPF) chickens at different doses, the immunized chickens were intradermally infected with 2000 plague forming unit (PFU) of duck embryo fibroblast (DEF) passaged SD2012-1, a very virulent Marek's disease virus. All chickens of the experiments and control chickens were observed daily throughout the entire experimental period to assess the vaccines efficacy and immunization dose against MDV SD2012-1 Strain. CVI988, CVI988+HVT and CVI988+SB1+HVT could partially protect immunized chickens against SD2012-1. CVI988+HVT and CVI988+SB1+HVT could provide more efficient protection than CVI988 at normal and high immunization dose. Excessive dilution of these vaccines could result in obvious decrease on protection against SD2012-1. In this study, 450 feather pulp samples collected from the dead or living chickens on Day 150 were detected to be MDV positive by polymerase chain reaction (PCR), and the positive rate of MDV was 100%.
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